| FEATURES
|
|
PRODUCT COMPONENTS:
Each vial contains 4 ul or 10ul of 2X concentration master mix. When
adding the template and primers, it results in 20ul or 50ul, containing
the following:
|
Contents
|
||
| Components |
20ul rxn
|
50ul rxn
|
| Thermostable DNA polymerase |
1 U
|
2.5 U
|
| dNTPs |
200 uM
|
200 uM
|
| MgCl2 |
2 mM
|
2 mM
|
| Stabilizer & tracking dye |
-
|
-
|
Experimental Protocol: Prepare PCR premix and add template DNA (5-50ng for 20ul rxn or 10-100ng for 50ul rxn) and primers (5-10pmole for 20ul rxn or 10-30pmol for 50ul rxn). Add water to make the final volume of 20ul or 50 ul.